Date: September 26, 2019. 12:00
Location: CCU Seminar Room
Title: Cellular Biographies: Reconstructing Developmental Trajectories
Affiliation: Harvard University, USA.
Complex gene expression programs determine the biographies of cells: how a cell divides, becomes specialized and differentiates. I will describe our recent efforts to use single-cell RNA sequencing and CRISPR-Cas9 genome editing to generate new tools to reconstruct differentiation trajectories and lineage trees at very large scales. Using mesoderm and brain development as examples, I will discuss the opportunities and challenges for these technologies to reveal the developmental biology of cells (also see McKenna et al. Science 2016; Farrell et al. Science 2018; Raj et al. Nature Biotechnology 2018).